APPLICATION OF QUANTITATIVE ANALYSIS IN TREATMENT OF OSTEOPOROSIS AND OSTEOARTHRITIS

“The math model of osteoporosis reveals a quick response to salubrinal and a delayed
but substantial response to knee loading”<-knee loading=LSJL

“the epiphyses of the proximal tibia and the distal femur are loaded, increasing intramedullary pressure in the adjacent bones. This pressure leads to interstitial fluid flow, which has been
shown to induce bone formation”<-interesting that the intramedullary pressure leads to the fluid flow and dismissing possible effects of the pressure itself.

Osteoperosis study:

C57BL/6 female mice (8 weeks old) were housed four to five mice per cage at the
Indiana University Animal Care Facility and fed with mouse chow and water ad
libitum.

“Loads were applied in the lateral-medial direction for 3 minutes/day at 15Hz, with peak-topeak
force of 0.5 N.”

“knee loading and salubrinal application elevated BMD via sclerostin and phosphorylated eIF2❛ (p-eIF2❛), respectively”

“Under 4-week salubrinal application, the BMD of ovariectomized mice decreased only 13%, with osteoblast activity increasing 136% while osteoclast activity decreased by 48%. After 4 weeks of knee loading, the BMD of ovariectomized mice decreased only 12%, with osteoblast
activity increasing 136% osteoclast decreasing 34%”

” loading’s suppression of p-eIF2❛ did not propagate to ATF4 and NFATc1.”

“ATF4 was modeled to increase the differentiation rate of osteoblast  precursors to active osteoblasts, while NFATc1 was modeled to increase the production of osteoclast precursors.”

” Loading was found to be more effective when applied earlier, as its effects are long term. Salubrinal seems to have a more immediate effect, thus being more advantageous when applied closer to the data collection time.”