How To Accelerate Osteanagenesis And Revascularization Of Tissue Engineered Bone In Big Animals

This is a study that will help me to at least develop a better understanding on the type of ideas and surgical methods that might be needed to make bone healing be possible if any distractions made is too large.

Analysis & Interpretation

The paper is in Chinese so the translated version to English is not as clear for a native English reader to understand. The basic premise is that the researchers got a bunch of goats, put them in 3 groups, and drilled a 2 cm wide hole in the tibia of the goats. The holes are then added with three different types of materials for each of the 3 groups to see how well the material added will be in helping the natural bone heal. The three types of implants used are…

  1. Coral Hydroxyapatite (CHAP)
  2. Coral Hydroxyapatite & Bone Marrow Stroma Cells (BMSCs)
  3. Coral Hydroxyapatite & Bone Marrow Stroma Cells & fascia flap (whatever this is)

The results showed that the first two groups saw either no or little bone healing. The 3rd group showed that the bone defect at around 2 cm wide still maanged to heal almost completely.

Implications for Height Increase

This paper was unique because we are still looking at the best type of fast bone healing/osteogenesis material. There may come a point where we choose to go with the bone distraction idea and if that is done, the defects made could be dramatic and we would need to look for a tissue engineered mixture to heal large bone defects purposely induced. We see from this post that the compound fascia flap seems to have the ability to induce vascularization for bone healing.

From PubMed study The method of accelerating osteanagenesis and revascularization of tissue engineered bone in big animal in vivo

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Feb;25(1):26-31.

[Article in Chinese]

Chen B, Pei GX, Wang K, Jin D, Wei KH, Ren GH.
Source

Department of Orthopaedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. chb@fimmu.com

Abstract

OBJECTIVE:

To study whether tissue engineered bone can repair the large segment bone defect of large animal or not. To observe what character the fascia flap played during the osteanagenesis and revascularization process of tissue engineered bone.

METHODS:

9 Chinese goats were made 2 cm left tibia diaphyseal defect. The repairing effect of the defects was evaluated by ECT, X-ray and histology. 27 goats were divided into three groups: group of CHAP, the defect was filled with coral hydroxyapatite (CHAP); group of tissue engineered bone, the defect was filled with CHAP + bone marrow stroma cells (BMSc); group of fascia flap, the defect was filled with CHAP + BMSc + fascia flap. After finished culturing and inducing the BMSc, CHAP of group of tissue engineered bone and of fascia flap was combined with it. Making fascia flap, different materials as described above were then implanted separately into the defects. Radionuclide bone imaging was used to monitor the revascularization of the implants at 2, 4, 8 weeks after operation. X-ray examination, optical density index of X-ray film, V-G staining of tissue slice of the implants were used at 4, 8, 12 weeks after operation, and the biomechanical character of the specimens were tested at 12 weeks post operation.

RESULTS:

In the first study, the defect showed no bone regeneration phenomenon. 2 cm tibia defect was an ideal animal model. In the second study, group of CHAP manifested a little trace of bone regeneration, as to group of tissue engineered bone, the defect was almost repaired totally. In group of fascia flap, with the assistance of fascia flap which gave more chance to making implants to get more nutrient, the repair was quite complete.

CONCLUSIONS:

The model of 2 cm caprine tibia diaphyseal defect cannot be repaired by goat itself and can satisfy the tissue engineering’s demands. Tissue engineered bone had good ability to repair large segment tibia defect of goat. Fascia flap can accelerate the revascularization process of tissue engineered bone. And by this way, it augment the ability of tissue engineered bone to repair the large bone defect of goat.

PMID: 12905602     [PubMed – indexed for MEDLINE]