Indirubin has shown promise before as a CXXC5 inhibitor. ” elongated tibial length through delayed senescence and further activation of the growth plate in adolescent mice.”<-further activation of the growth plate is what is most interesting.

We have to be on the lookout for indirubin derivative, KY19382.

Here’s a patent PHARMACEUTICAL COMPOSITION CONTAINING INDIRUBIN DERIVATIVE AS ACTIVE INGREDIENT

Interesting parts of the patent:

“The composition of claim 7, wherein the compound promotes bone growth and/or increases bone thickness.

12. The composition of claim 7, wherein the compound promotes chondrocyte proliferation, induces differentiation of osteoblasts, and/or activates β-catenin stabilization and nuclear translocation of β-catenin.”

“The composition for promoting bone length growth in accordance with the present invention has the promoting effects on both proliferation of chondrocytes and differentiation of osteoblasts, thereby increasing both bone length and thickness (bone density). Therefore, because it has the advantages of a wide range of prescriptions for various age groups, it can be usefully used as a pharmaceutical composition that can promote the growth of bone length and thickness.”

“The food compositions of invention, if manufactured as a beverage, is essential at the rate indicated, and there is no specific restriction on the liquid composition, such as ordinary drinks, and may contain several flavoring agents or natural carbohydrates as an additional ingredient. Examples of natural carbohydrates described above are monosaccharides (e.g. glucose, fructose and the like), disaccharides (e.g. maltose, sucrose and the like), and polysaccharides (e.g. dextrin and cyclodextrin, as well as common sugars such as xylitol, sorbitol and erythritol). As flavoring agents other than those described above, natural flavoring agents (e.g. taumatine, stevia extracts, such as fume-based A and glycirrhizine, and synthetic flavoring agents, such as sakarin, aspartame) can be advantageously used. The ratio of the above natural carbohydrates is generally about 1 to 20 g per 100 ml of the composition of this invention and, preferably, about 5 to 12 g.”

So seems like it will be a beverage which could mean it’s for everyone!!!!!!!!!!

Here’s another interesting study:

Indirubin-3′-oxime stimulates chondrocyte maturation and longitudinal bone growth via activation of the Wnt/β-catenin pathway

“we identified indirubin-3′-oxime (I3O) as a compound capable of enhancing longitudinal bone growth. I3O promoted chondrocyte proliferation and differentiation via activation of the Wnt/β-catenin pathway in vitro. Intraperitoneal injection of I3O in adolescent mice increased growth plate height along with incremental chondrocyte maturation. I3O promoted tibial growth without significant adverse effects on bone thickness and articular cartilage. Therefore, I3O could be a potential therapeutic agent for increasing height in children with growth retardation.”

Look at the tibia growth in fig2 at 5uM it is sick!  Although a bit curved.  If you look at 2a it looks fairly normal though.

“I3O significantly increased the mRNA levels of early chondrogenesis markers, such as Col2a1 and Sox9. In addition, I3O treatment increased the mRNA levels of fully differentiated hypertrophic chondrocyte markers, such as Col10a1, Vegfa, Alp, and Mmp13, as well as prehypertrophic chondrocyte markers, such as Runx2 and Mmp9. Transcriptional induction of these genes by I3O treatment was suppressed by knockdown of Ctnnb1″

“The practical usage of I3O is advantageous because it is an indirubin derivative that is the main active ingredient in a traditional Chinese medicine, Danggui Longhui Wan”<-even if indirubin is in the Danggui Longhui Wan we still don’t know if it’s effective.

All height seekers should be monitoring Indirubin

Here’s a biohacker thread on indirubin.  One interesting comment: “The issue seems to be that the drug has not been studied in humans, nor in vivo for this application. With the current understanding of hormono-dynamics, GH secretagogues + antidiabetic compounds (metformin) seem to be the best bet to enhance height safely in adolescents.”  On metformin “Metformin in this case would mediate any deleterious effects on glucose metabolism exerted by the gh secretagogues which are known to mess up glucose tolerance.”

Here’s some studies with updates on Indirubin:

ERα/β/DMP1 axis promotes trans-differentiation of chondrocytes to bone cells through GSK-3β/β-catenin pathway

On the LSJL forums someone mentioned an anecdotal case where an individual supplementing with C60 fullerene got taller.  Does mentioned was about 50-100mg/year.  Several supplements were used though.  But in our quest to go taller it is worth to look at every single compound in existence to see if it could potentially have height increase improvements.  This page says C60 fullerene has been found to improve longevity, promote cartilage and bone strength, and result in less stiffer joints.  Since bone growth cessation is a form of aging we want to look at any anti-aging compound.  Obviously, anything that affects cartilage and bone is of interest to us.

Here’s the original post on the height increase.  The author speculates the the height increase may be due to “C60 is used with stearic acid to increase the pool of stem cells.”  “C60 stimulates mitochondria, and mitochondrial activity drives the activity of stem cells.”

According to this

Water-soluble C60-(OH)24 fullerene hydroxide as a therapeutic agent against the degeneration of articular cartilage in osteoarthritis

“During the development of osteoarthritis (OA), mechanical stress on articular cartilage downregulates the stable cellular activities of chondrocytes and induce the production of catabolic factors, such as proinflammatory cytokines and chemokines. In addition, it is well known that chondrocytes produce excess amounts of reactive oxygen species (ROS; superoxide, nitric oxide, hydrogen peroxide and peroxynitrite) in response to mechanical stress. Recent reports have demonstrated that mechanical stress to articular cartilage stimulates excess production of ROS from chondrocytes, consequently resulting in the depolymerization of hyaluronic acid and chondrocyte death. It has been suggested that the generation of ROS and the depletion of cellular antioxidants may be involved in the pathogenesis of OA.”

Since fullerene is an anti-oxidant it could potentially reduce height loss as a result of aging by preventing height loss due to Reactive Oxygen Species.  Since the individual mentioned in the forum is 60 years old that is a possibility.

“IL-1β significantly inhibited the production of proteoglycan and the glucose uptake in chondrocytes, and accelerated the secretion of MMP-3 from chondrocytes. Even in the presence of IL-1β (1.0 ng/ml, but not 10.0 ng/ml), C60(OH)24 inhibited the IL-1β-induced production of MMP-3, the IL-1β-induced down-regulation of proteoglycan production and glucose metabolism in OA chondrocytes. C60(OH)24 fullerene hydroxide also reduced the OA-relating catabolic factor-induced up-regulation of DNA repair enzyme, Apex 2, in OA chondrocytes.”

C60 is also used as a matrix for cartilage transplants according to Biodegradable polymers in chondrogenesis of human articular chondrocytes,

According to

Biological functions of fullerene

“A mixed aqueous solution of C60 and poly(vinyl pyrrolidone) is reported to induce differentiation of chondrocytes”<-this is a potential height increasing application if it can occur just by taking it naturally in the human body.

There are several studies that study that fullerene can induce cartilage differentiation and prevent cartilage destruction.

A Novel Promoting Action of Fullerene C60 on the Chondrogenesis in Rat Embryonic Limb Bud Cell Culture System

“Fullerene C60 was solubilized with polyvinylpyrrolidone (PVP) in water, and the aqueous solution was applied to the rat limb bud cell differentiation system. By the incubation with various concentrations of C60 with poly(vinylpyrrolidone) , cell differentiation was extremely promoted,up to the levels of a 3.2-fold increase of the controls. Although poly (vinylpyrrolidone) alone inhibited the cell differentiation in proportion to the concentration, the observed promoting action by C60 surpassed the action of poly(vinylpyrrolidone). This specific promoting action on the chondrogenesis is the novel significant activity of C60.”

The study speculates that C60 may promote chondrogenesis by resulting in the condensation of chondroitin sulfate which in turn promotes chondrogenesis.

The results on fullerene are inconclusive as we can’t be sure how the individual got the height(articular cartilage growth or increase in bone length?) and whether the height was due to reduced cartilage catabolism or due to stem cell proliferation.  And there’s not enough human studies.  One inch isn’t a lot to get excited about either.

Any loading study with pictures can provide insight on the possibility of ectopic chondrogenesis/endochondral ossification even if they do not directly study longitudinal bone growth as long as there are images.

One method to grow taller as adult:

1)Induce bone degradation allowing space for new growth plates to form(one way this could be induced is by inducing fluid flow flow in the bone via joint loading)

2) stimulate new growth plate formation via mesenchymal condensation(this would be induced by hyrostatic pressure via joint loading)

3) induce endochondral ossification of the ectopic cartilage.  This would happen naturally due to the bone microenvironment.

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β‐catenin signaling

“Osteoporotic osteoarthritis (OPOA) is a common bone disease mostly in the elderly, but the relationship between Osteoporotic (OP) and osteoarthritis (OA) is complex. It has been shown that knee loading can mitigate OA symptoms. However, its effects on OPOA remain unclear. In this study, we characterized pathological linkage of OP to OA, and evaluated the effect of knee loading on OPOA. We employed two mouse models (OA and OPOA), and conducted histology, cytology, and molecular analyses. In the OA and OPOA groups, articular cartilage was degenerated and Osteoarthritis Research Society International score was increased. Subchondral bone underwent abnormal remodeling, the differentiation of bone marrow mesenchymal stem cells (BMSCs) to osteoblasts and chondrocytes was reduced, and migration and adhesion of pre‐osteoclasts were enhanced. Compared to the OA group, the pathological changes of OA in the OPOA group were considerably aggravated. After knee loading, however, cartilage degradation was effectively prevented, and the abnormal remodeling of subchondral bone was significantly inhibited. The differentiation of BMSCs was also improved{this is the kind of thing we would be looking for}, and the expression of Wnt/β‐catenin was elevated. Collectively, this study demonstrates that osteoporosis aggravates OA symptoms. Knee loading restores OPOA by regulating subchondral bone remodeling{subchondral bone remodeling could potentially increase height by increasing bone deposition at the longitudinal ends of bone}, and may provide an effective method for repairing OPOA.”

“Since bone is a mechano‐sensitive organ, moderate pulsating loading, oscillatory compressive loads, can be applied to various synovial joints in a form of elbow loading, knee loading and ankle loading. Joint loading can change intramedullary pressure of bone cavities. The moderately changing pressure may generate fluid flow in a lacuna canalicular network in bone cortex, and activate anabolic genes in bone{the goal would that a change in hydrostatic pressure in the bone would stimulate ectopic chondrogenesis/endochondral ossification}. In our previous studies, we have shown that knee loading is effective in …promoting longitudinal bone growth…”

“Dynamic loads were sinusoidal at 1 N (peak‐to‐peak) and the frequency was 5 Hz for 6 min/day (each knee, 3 min).”

It’s here that we’d look for signs for ectopic chondrogenesis.  It’s hard to tell for sure as there’s no loading group for normal bone.  In the the loading groups(OAL, OPOAL) there are clear differences in the subchondral bone plate.  Although the loading groups, all seem to have differences in the bone but the differences seem stronger in the trabecular bone.  The growth plate is different in the loaded groups but that’s not going to make a difference to adults.

In this it looks like there may be more cellular condensation in the OAL group and the OPOAL group(there are more cells and they’re closer together).  This is a precursor to mesenchymal chondrogenesis.  We’d need signs of bone degradation as well to prove that growing taller as an adult is possible.  And it’s hard to say that without seeing a normal bone.

Note:  I am still working on a new method to increase height.

Sometimes things that are good for bone development are bad for longitudinal bone growth.  Fluoride could be such a case.  We can’t say definitively that fluoride stunts growth but it’s definitely something to watch out for.  Apparently, there is some concern that babies can have too much fluoride which makes sense given their small body size.

It should be noted also that sometimes things that inhibit longitudinal bone growth in one bone can stimulate in another.  This can occur in the spine for example.

Fluoride Inhibits Longitudinal Bone Growth by Acting Directly at the Growth Plate in Cultured Neonatal Rat Metatarsal Bones

“Excessive intake of fluoride inhibits bone growth in both humans and animals. It is unknown whether fluoride acts directly on the growth plate to inhibit longitudinal bone growth, and its mechanism of action has not been elucidated. In this study, we used an organ culture system and SW1353 cells to evaluate the effects of fluoride on endochondral ossification. Neonatal rat metatarsal bones were dissected and cultured with or without fluoride for 7 days. The total length and width of the metatarsal rudiments and the length of the calcification zone were measured. Chondrocyte proliferation, differentiation, and apoptosis were analyzed by immunohistochemistry and TUNEL assay in sectioned bones. The apoptosis was detected by flow cytometry, and the expression of apoptosis-related proteins Bax, Bcl-2, and Caspase-3 were detected by western blotting in SW1353 cells. Linear measurements demonstrated that fluoride induced a biphasic effect on longitudinal bone growth in organ culture, with a significant growth inhibition at a high concentration (10⁻⁴ M) and a stimulatory action at low concentration (10⁻⁶ M) of fluoride{that’s a pretty significant difference between high and low concentration so you can pretty safely take fluoride}. Histomorphometrical analysis of growth plate from fluoride-exposed metatarsal rudiments showed a significant reduction in the height of the proliferative and hypertrophic chondrocyte zones. Analysis of the Col2α1 and Col10α1 expression by immunohistochemistry revealed fluoride-suppressed metatarsal growth plate chondrocyte proliferation and differentiation. In addition, fluoride increased the number of apoptotic chondrocytes in the metatarsal growth plate. Western blotting showed an up-regulated expression of Caspase-3 and Bax and down-regulated expression of anti-apoptotic protein Bcl-2 after treatment with 5 × 10⁻⁴ M fluoride in SW1353 cells. Our findings indicated that fluoride inhibited longitudinal bone growth by acting directly at the growth plate in cultured neonatal rat metatarsal bones. Such growth inhibition was mediated by suppressing proliferation and differentiation, increasing apoptosis of resting chondrocytes and causing premature cell senescence in the growth plate.”

If you read the paper it says that high doses of fluoride can enlarge the growth plate which indicates that an enlarged growth plate is not always beneficial for longitudinal bone growth.

In the paper it says 10 -6 M of fluoride increased Longitudinal bone growth but it was not statistically significant but any longitudinal bone growth is good.

The question is can fluoride in toothpaste, drinking water, and other dental sources reach high concentration and stunt growth?

This is a Hiroki Yokota study who along with Ping Zhang is one of the prime developers of lateral loading modalities.

Generation of the Chondroprotective Proteomes by Activating PI3K and TNFα Signaling

“Chondrosarcoma and inflammatory arthritis are two joint-damaging diseases. Here, we examined whether a counterintuitive approach of activating tumorigenic and inflammatory signaling may generate joint-protective proteomes in mesenchymal stem cells and chondrocytes for the treatment of chondrosarcoma and inflammatory arthritis{we already kind of know that low levels of inflammatory signaling is beneficial, it’s too much that’s the problem}. While activating PI3K signaling and the administration of TNFα to chondrosarcoma cells and chondrocytes promoted tumor progression and inflammatory responses, those cells paradoxically generated a chondroprotective conditioned medium. Notably, the chondroprotective conditioned medium was enriched with Hsp90ab1 that interacted with GAPDH. Extracellular GAPDH interacted with L1CAM, an oncogenic transmembrane protein, and inhibited tumorigenic behaviors, whereas intracellular GAPDH downregulated p38 in chondrocytes and exerted anti-inflammatory effects. The result supports the unconventional approach of generating chondroprotective proteomes.”

“We employed mesenchymal stem cells and chondrocytes to generate chondroprotective proteomes{proteomes are essentially a collection of proteins} by activating PI3K signaling and the administration of TNFα.”

“In response to chemotherapeutic agents, the reverse behavior of resilient cancer cells is frequently observed. While their tumorigenic action may initially be suppressed, cells tend to develop drug resistance and gradually strengthen their progression”

“Chronic inflammation is reported to facilitate tumor progression, while the induction of inflammation may contribute to suppressing tumorigenesis by stimulating immune responses”

“Besides chondrocyte-derived CM, MSC-derived CM, as well as MH7a synovial cell-derived CM, were converted to tumor suppressive by the treatment with YS49, an activator of PI3K signaling. Their application reduced the level of TNFα and MMP13 in C28/I2 chondrocytes”

“Hsp90ab1, a heat shock protein acting as a molecular chaperon, showed the most pronounced anti-inflammatory effect in C28 chondrocytes by downregulating TNFα and MMP13”

” the chondroprotective proteome can be generated from MSCs and chondrocytes by activating PI3K signaling with YS49 and treating them with TNFα. When switched on, both PI3K and TNFα signalings acted tumorigenic in CS cells and inflammatory in chondrocytes. However, YS49 and TNFα converted skeletal host cells such as MSCs and chondrocytes into iTSCs and iISCs, and they generated tumor-suppressive and inflammation-suppressive CMs. We observed that YS49 and TNFα-treated CMs inhibited the proliferation, migration, and invasion of CS cells. Those CMs downregulated Runx2 and MMP9 in CS cells, as well as NFATc1 and cathepsin K in osteoclasts, whereas they decreased TNFα and MMP13 in chondrocytes”

Natural Height Growth has written about Ginza Kojima’s devices before.  The device appeared to be some kind of traction device with a rotational force applied.  The centripetal force could potentially stretch the body.  But all these forces ultimately have to lead to some kind of plastic deformation(permanent stretch of the bone or cartilage).  I speculated previously that the green light may be some kind of radiation that weakens the bone which allows it to be permanently stretched.

Here’s Ginza Kojima’s new website: http://www.shincho-nobashi.jp/

It looks like it applies a rotational/twisting force on the leg combined with some kind of pressure.

It looks like it’s mainly for small amounts of height as taken by this excerpt below.

”

In the case of the whole body course (guarantee course), it is 500,000 yen up to 1 cm, 1,300,000 yen up to 2 cm, and 2,300,000 yen up to 3 cm. (Excluding tax ※ Consumption tax will apply separately)

In the case of the knee and foot bone course (guarantee course), it is 1 million yen to 1 cm, 2 million yen to 2 cm and 3 million yen to 3 cm. (Excluding tax ※ Consumption tax will apply separately)

Please inquire for detailed charges for 3 cm and beyond.”

One limb length testimonial though advertised in the site is for 5 cm which you cannot realistically achieve without actual major bone growth.

”

The effects of the treatment are very individual differences and it is not known how long it will take without actually starting the treatment.

In our hospital, we guide you to receive the treatment once a week 24 times (about 6 months) as a rough guide. In addition, we offer a course that you can receive intensive treatment for a week as a short-term intensive course, so please consult with distant people.”

<-so looks like once a week for 6 months.

The current treatment schedule at our hospital is as follows.

• 1st time 10:00 to 12:00 (30 minutes set time)
• Second time 12:30-14:00
• Third time 14:30-16:00

As mentioned above, an average of 3 sets is given daily. The second and subsequent treatments are basically the same, but we will make adjustments to suit your convenience as much as possible, so please contact us.”<-and 3 sessions of 30 minutes.

This machine advertised elsewhere on the site looks like a traction machine

Gina kojima explains the science behind his thinking here:

“First of all, I thought it would be good to pull. When I grabbed the bones of the foot from both sides and pulled it with a slight force according to the tug of war and the principle of childhood, I thought that it would be fine because the blood vessels became thinner and blood flow would improve. The heart is pumping 6 liters of blood a minute, so the blood vessels become thin if you pull a round blood vessel, and it is the theory that so much pressure will be applied to improve blood flow. It has been 40 years since I started making machines. To be honest, it will be dozens already. Anyway, it is said that if you look at it, why do you spend so much money to make stupid tools, and it keeps making it anyway.”

So this quote makes it seem like a traction device but a lot of traction devices don’t necessarily pull the bone to induce plastic deformation.

As far as traction devices go, you have to either induce plastic deformation in the bone or you have to stimulate the body to grow.

I think the whole body course is the traction machine and the knee course is the elevated height speed machine 2 device.

I think elevated height speed machine 2 device may just be another traction device but specifically for the knee.  Any japenese translators?