Hyperostosis indicates that it’s possible to form new bone as an adult.  Hyperostosis seems to affect ligaments which would make it hard for height growth as there ligaments really placed to add to height.  It may be linked to insulin as diabetes may affect hyper ostosis.

Morphological characteristics of diffuse idiopathic skeletal hyperostosis in the cervical spine.

“(A) Plain lateral radiograph shows a 69 year old male with DISH in the cervical spine. A solid formation of new bone is extending over at least four vertebral bodies. (B) Computed tomography (CT) visualizes the thoracic spine of a 72 year old male in the sagittal view. The scan shows a flowing ossification of the anterolateral spine with bridging over more than four contiguous vertebral bodies. The intervertebral discs and apophyseal joints are relatively intact in both images. (C + D) The CT scans in axial view demonstrate the differences in position of the new bone formation depending on the region. (C) The CT scan of the cervical spine corresponds to the radiographic image (A) and demonstrates symmetrical hyperostosis (yellow) anterior to the vertebral body and possible displacement of the trachea. (D) The axial CT of the mid thoracic spine in a 58 year old male with DISH shows the newly formed bone on the right anterolateral side with the aorta clearly located on the left anterolateral side.”<-So this is all over time.

“It has been hypothesized that vascular structures act as a natural barrier for the formation of new bone in DISH”

If blood vessels do block bone growth than that fact can be exploited to increase height.  There is an epiphyseal artery though that doesn’t block growth.

“arteries may act as a natural barrier for newly formed bone in DISH. The lack of crossing segmental vessels in the cervical spine may permit linear bone growth creating a bar-like structure in contrast to the flowing pattern typical for DISH in the thoracic spine where segmental vessels are present.”

Some people have claimed to have grown taller in a non-epiphyseal plate driven method via HGH.  Now it’s important to note that acromegaly is not solely based on HGH and there are some cases in gigantism where HGH levels are lower than people who supplement via HGH so it’s possible there’s another factor driving the length.  This topic was discussed before here.  In that page there are additional acromegalic x-rays and additional discussion of non-growth plate driven height growth.

You don’t just grow interstitially, you also grow appositionally even on the longitudinal ends of the bones.  For most, this is insignificant but for someone with high HGH and thereby higher bone turnover this could be much more significance but the question then becomes if they are gaining height in the feet and hands due to the larger number of bones there then in legs and arms then why aren’t the growing in the torso.

It follows logically that if two people are growing by different methods interstitial growth(traditional growth plate growth) and appostional growth on the longitudinal ends(endochondral ossification of the articular cartilage or some other bone thickening method) then the two bone shapes will look differently on the x-ray.

Here’s a “normal” hand x-ray:

Here’s an acromegalic hand x-ray:

One thing that strikes immediately is the greater whiteness between the acromegalic x-rays and the normal x-rays but the two bone shapes seem largely the same.  However there does seem to be greater articular cartilage spacing which could explain the increased hand size.

I couldn’t find a spine xray of someone with acromegaly but here’s a chest x-ray:

Unfortunately you can’t really tell anything about the spine because the bones are so thick.

Here’s a normal chest x-ray:

So basically the x-rays tell you that acromegaly may or may not cause a form of non-growth plate based longitudinal bone growth.  There’s just not enough x-rays of people with acromegaly to draw conclusions.  Or x-rays of people who supplement with HGH like Richard Piana.

Here’s a study(Unfortunately I couldn’t get the full study) that may have some insights:

Acromegaly and bone.

” Growth hormone (GH) and insulin-like growth factor-I (IGF-1) have pleiotropic effects on the skeleton throughout the lifespan by influencing bone formation and resorption. Despite these positive effects on skeletal metabolism, in presence of GH and IGF-1 excess, bone turnover increases excessively leading to deterioration of bone microarchitecture and high risk of fragility fractures, thereby impairing quality of life.

Coexistent hypogonadism, diabetes mellitus, hypovitaminosis D, hyperparathyroidism and over-replacement with glucocorticoids impair bone framework, however, the effects of acromegaly on bone mineral density (BMD) are still controversial and despite normalization of bone turnover after treatment, the risk for fractures remains increased. As a matter of fact, a major clinical aspect emerging from the studies published so far is the lack of clinical-diagnostic tools able to reliably predict the appearance of fractures in patients with acromegaly occurring even in the presence of normal or low-normal BMD.”

So bone turnover could potentially alter bone architecture and make non-growth plated based growth a possibility.

eIF2alpha signaling regulates ischemic osteonecrosis through endoplasmic reticulum stress

This is the effect of salubrinal on normal animals.  You can see salubrinal makes the bone more porous in the image.

14 Week old rats were used.

The dosage was 0.5mg/kg of salubrinal for 4 weeks.  If salubrinal can make bone more porous than it has the potential to be used for longitudinal bone growth.  Ping Zhang and Hiroki Yokota two LSJL scientists are putting a lot of effort into this so it is possible that there are effects of Salubrinal that they haven’t mentioned yet.

Here’s a hydrostatic pressure study that I found:

Microdamage assessment in equine bone resulting from high hydrostatic pressure and/or irradiation.

“HHP (~1200 MPa) was applied to non-irradiated and irradiated specimens of equine cortical bone and compared to non-pressurized controls”

“Twenty four cylindrical specimens (nominally 6.35 mm in diameter and 12.7 mm in length) were machined from the mid-diaphysis of an equine third metatarsal such that the longitudinal axis of the specimen cylinder and metatarsal were coincident. Specimens were kept hydrated throughout the machining process. Six specimens were then assigned to each of four groups: +HHP/-IR, +HHP/+IR, -HHP/-IR and -HHP/+IR (+ and – denote presence and absence, respectively). HHP was applied using a modified hydrostatic extrusion press  in the manner consistent with much other work. Specimens were immersed in silicone oil at 37°C, pressurized to the desired level and immediately depressurized. In this group of specimens, 1200 MPa was the minimum pressurization value. “<1200MPa is extraordinarily high.

“microcracks were found in all fields of all specimens and ranged from 0.1 to 0.375 mm across groups. In the +HHP/+IR group, on average, 14% of the microcracks had lengths greater than 0.1 mm however in all other groups microcracks of this length comprised only 1 to 4% of the identified microcracks. The vast majority of microcracks were situated within the interstitial tissue and often in a parallel assemblage between osteons. Microcracks were found to travel along cement line interface around the osteon in just the pressurized specimens regardless of the presence or absence of irradiation. Only rarely were microcracks seen to progress across cement lines and/or lamellae ”

Figure 1 shows the microcrack images.

Here’s one with 350MPa HP.

Biomechanical investigation of the effect of high hydrostatic pressure treatment on the mechanical properties of human bone

“The bone specimens from one side were exposed to different pressure values of 300 or 600 MPa over 10 min.”

“Biomechanical properties of the cortical and trabecular bone did not decrease after exposure to 300 MPa regarding the testing parameters Young’s modulus and ultimate strength ”  The study did not show any cortical bone damages so we can’t say whether there was or was not.

“Even after HHP treatment at 600 MPa the strength of bone only decreases up to 15%.”

So High Hydrostatic Pressure does damage cortical bone and cortical bone is restrictive to longitudinal bone growth but we will never be able to safely generate that much pressure in the bone.

Here’s the last LSJL update.

Here’s the latest feet images

One effect that clamping my right foot has had is that my right quad is a lot bigger than my left quad now.  I left weights as muscle should help with LSJL as muscle can contract to stimulate bone and generate hydrostatic pressure.  Differences in feet size change weight distribution.  So now I’m going to clamp my left foot and not my right foot to try to get it to catch up.  If I can bridge the gap between the two that’ll be strong proof of LSJL.

Here’s a picture from another angle that shows the size difference better:

The right calcaneus is also bigger so if I get some left foot growth I’ll gain some small but significant amount of height.

I may have gained some arm length but it’s within measurement error.

I don’t think clamping the toes is what’s helping.  I think it’s the clamping of the feet itself.  I am doing hand clamping as well.  I am clamping individual fingers and pretty much the whole hand is growing and not the specific fingers.  I have x-rays showing that my left hand fingers were longer except for the metacarpal.  So if I can’t get my left foot to catch up that’ll be another option to prove LSJL.  If my right hand keeps growing significantly I can get x-rays.

We’ll see how things go with my left foot.  It seemed I was almost ready to go up a shoe size with my right foot but with the muscle imbalance I’ll have to go with my left and get them to equalize.

I actually missed that this LSJL study showed height increase as it was a minor comment that joint loading increased height of the femoral head.  Since the femoral head is a diagonal offshoot of the femur it may not necessarily increase height but it is an increase in bone length all the same.  However, the osteonecrosis induced in the study may be a prerequisite to induce the femoral head growth.  However, it should be noted that the osteonecrosis decreased femoral head height but the degradation of existing bone may have allowed for new bone growth.

This is an LSJL related study published by Yokota and Zhang.  The primary author seems to be more Zhang who seems more into the lengthening effects than Yokota.  This study shows that joint loading increases fibrous differentiation.  And fibrous differentiation would be a potential intermediary step for neo-growth plate formation.  Knee loading increased vessel remodeling and osteoclast formation which would be needed to make room for a new growth plate but these levels were only slightly greater than control group and reduced from the osteonecrosis group so we can not say for sure whether this will happen in LSJL on a normal bone.

Overall this study shows that LSJL does at least one of the steps required for neo-growth plate formation: fibrous type tissue formation.  This fibrous tissue would then have to be further differentiated into more cartilagenous tissue.  The perichondrial ring(or ring of LaCroix) is fibrocartilagenous.

Knee loading protects against osteonecrosis of the femoral head

“Osteonecrosis[loss of blood to the bone] of the femoral head is a serious orthopedic problem. Moderate loads with knee loading promote bone formation.  Using a rat model, we examined a hypothesis that knee loading enhances vessel remodeling and bone healing through the modulation of the fate of bone marrow-derived cells. Osteonecrosis was induced by transecting the ligamentum teres followed by a tight ligature around the femoral neck. For knee loading, 5 N loads were laterally applied to the knee at 15 Hz{this is pretty high frequency} for 5 min/day for 5 weeks. Changes in bone mineral density (BMD) and bone mineral content (BMC) of the femur were measured to evaluate vessel remodeling. Femoral heads were harvested, and bone marrow-derived cells were isolated to examine osteoclast development and osteoblast differentiation.  Osteonecrosis significantly induced bone loss, and knee loading stimulated both vessel remodeling{vessel remodeling shows promise as that would be very helpful for neo growth plate creation} and bone healing. The osteonecrosis group exhibited the lowest trabecular BV/TV in the femoral head, and lowest femoral BMD and BMC. Knee loading increased trabecular BV/TV as well as BMD and BMC. Osteonecrosis decreased the vessel volume, vessel number and VEGF expression, and knee loading increased them. Osteonecrosis activated osteoclast development, and knee loading reduced its formation, migration, adhesion and the level of “pit” formation{pit formation could potentially be beneficial though as that pit could where a neo-growth plate would go}. knee loading significantly increased osteoblast differentiation and CFU-F{An increase in CFU-F means an increase in mesenchymal stem cell proliferation which is a good for neo-growth plate formation but doesn’t guarantee that it will occur}. A significantly positive correlation was observed between vessel remodeling and bone healing. Knee loading could be effective in repair osteonecrosis of the femoral head in a rat model [by] promoting vessel remodeling, suppressing osteoclast development, and increasing osteoblast and fibroblast differentiation. “

“The mechanism of knee loading is considered to change intramedullary pressure of femoral and tibial bone cavities. The load driven pressure may generate fluid flow in a lacuna canalicular network in bone cortex. The pressure activates bone metabolism-related genes in femur and tibia”<-what we are interested in an increase in fluid flow and hydrostatic pressure to induce chondrogenic differentiation.  Hydrostatic pressure by itself isn’t likely to induce chondrogenic differentiation by itself as it is typically three orders of magnitude below the pressure needed to induce chondrogenic differentiation.  But the addition of bone deformation and fluid flow may bridge the gap and induce chondroinduction.

“Male Sprague–Dawley rats (~12weeks of age)”

“knee loading was achieved through dynamic loads applied to the left and right knee joints of rats in the lateral–medial direction, respectively. To position the knee properly, the lower end of the loading rod and the upper end of the stator were designed to form a pair of semispherical cups. The lateral and medial epicondyles of the femur together with the lateral and medial condyles of the tibia were confined in the cups.  The tip of the loader had a contact area of 15 mm in diameter.”

See Figure 1 of the paper(first link on the page) for an image of the knee loader.

Loading increased vascular remodeling in the osteonecrosis + LSJL group but not versus control.  But there was no normal bone plus loading group.

Joint Loading actually seemed to reduce the number of mesenchymal stem cells but that could be due an increase in differentiation.  Mesenchymal condensation was more visible in the osteonecrosis group and mesenchymal condensation is a prerequisite for neo-growth plate formation.

Knee Loading seemed to restore osteoclast adhesion and migration to slightly above control levels but that slightly above could indicate activity that would remodel the bone enough to allow for mesenchymal condensation.

Knee loading greatly increased CFU-F and Osteoblast differentiation versus both osteonecrosis and control group. “Knee loading enhanced differentiation of osteoblasts and fibroblasts.” Fibroblastic tissue(marked by CFU-F) could potentially become chondrogenic tissue.  Fibrocartilage is a thing.  But to be a true growth plate, additional mechanical stimulation would be needed to turn that fibrocartilage into pure cartilage.

” Knee loading increased the height of the femoral head partially”

It’s a big breakthrough that LSJL increased height in the femoral head.  The osteonecrotic bone had far more marrow in B, thus under osteonecrosis LSJL may have had more room to induce growth.

The perichondrial ring of the growth plate is fibrocartilagenous in nature and may be the source to provide cells to the growth plate.

“The current histology and bone mineral density data are suggestive of the role of bone marrow-derived stem cells in load-driven bone healing, and they also establish a causal relationship between the observed vessel remodeling and bone healing effects with knee loading”<-This is huge because we want to induce stem cells to form neo-growth plates.

According to this LSJL would increase blood perfusion which would be the most interesting thing.  If the bone has more blood flowing through can create unique biological opportunities remember one of the key steps to distraction osteogenesis is a blood clot.

It was mentioned that LSJL upregulates bone metabolism related genes.  Here’s a study that lists bone metabolism related genes:

Association of osteoporosis susceptibility genes with bone mineral density and bone metabolism related markers in Koreans: the Chungju Metabolic Disease Cohort (CMC) study.

“bone metabolism-related markers, such as serum concentrations of calcium, phosphorus, PTH,
and 25(OH) D”

“genetic variants of MEF2C, ESR1, TNFRSF11B, and SOX6 were associated with bone metabolism-related markers”

Here’s the last LSJL update.

The growth seems pretty solid from last update:

Current

1 month earlier:

So increasing the intensity and duration of clamping on the right foot seems to have worked.  I’ll keep trying to see if can get more growth.  I may have some growth in my right hand from clamping there but I’ll wait to see if the growth is more significant as I can always get x-rays there.

I’ll also try to get growth in the longer limbs but once I can prove growth in smaller limbs I can get more resources to use for the longer ones.  It does seem like the foot itself is growing more than the toes so maybe it’s easier to grow those bone shapes even though there’s a lot of the same obstacles for longitudinal bone growth for “short” bones there could be differences we don’t know about.  these bones are also surrounded by different tissues than long bones which could make a difference.

So I’ll try the same intensity/density of clamping on the foot and see if I can grow on the hands and other limbs and increase clamping intensity on those areas.